What are the DOT urine specimen collection procedures under 49 CFR Part 40?

Short answer

The DOT urine specimen collection procedures under 49 CFR Part 40 require a collection site meeting specific security and facility requirements, a trained collector, and adherence to a detailed protocol for collecting and handling the specimen. Four things are key: the collection site, the collector, the collection process, and the materials used.

The rule says

"A urine collection for a DOT drug test must take place in a collection site meeting the requirements of this section." (49 CFR 40.42(a))

"Collectors meeting the requirements of this subpart are the only persons authorized to collect urine specimens for DOT drug testing." (49 CFR 40.31(a))

"For each DOT drug test, you must use a collection kit meeting the requirements of Appendix A of this part." (49 CFR 40.44)

"As the collector, you must take the following steps before the employee provides the urine specimen" (49 CFR 40.63)

In plain English

The collection site must provide privacy and security for the employee, with a single-toilet room or a multistall restroom that meets specific requirements. The collector must be trained and follow a detailed protocol to ensure the integrity of the specimen. This includes verifying the employee's identity, providing a secure collection container, and observing the employee during the collection process to prevent tampering. The collector must also follow specific procedures for handling and shipping the specimen to the laboratory.

What to do

• Ensure the collection site meets the requirements of 49 CFR 40.42
• Use a trained collector who meets the requirements of 49 CFR 40.31 and 40.33
• Follow the detailed protocol for collecting and handling the specimen outlined in 49 CFR 40.63
• Use a collection kit that meets the requirements of Appendix A of 49 CFR Part 40, as specified in 49 CFR 40.44