Ask X3What does 49 CFR 40.71 (How does the collector prepare the urine specimen?) require?
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According to 49 CFR 40.71, all collections under DOT agency drug testing regulations must be split specimen collections. After the employee brings the urine specimen to you, you must take the following steps in order, in the presence of the employee:
1. Check the CCF: Check the box on the CCF (Step 2) to indicate it was a "Urine" and "Split" specimen collection.
2. Pour the primary specimen: You (not the employee) must pour at least 30 mL of urine from the collection container into the primary specimen bottle.
3. Pour the split specimen: You (not the employee) must pour at least 15 mL of urine from the collection container into the second (split) specimen bottle.
4. Secure the lids: You (not the employee) must place and secure (tighten or snap) the lids or caps on both bottles.
5. Seal the bottles: You (not the employee) must place tamper-evident bottle seals over the caps/lids and down the sides of the bottles.
6. Date the seals: You (not the employee) must write the date on the tamper-evident bottle seals.
7. Get employee initials: Ensure the employee initials the tamper-evident bottle seals to certify the bottles contain their specimens. If the employee refuses or fails to initial, note this in the "Remarks" line of the CCF (Step 2) and finish the collection process.
8. Discard leftover urine: Discard any urine left over in the collection container. The only exception is that you may use excess urine for clinical tests (like protein or glucose) if the collection is done alongside a physical exam required by a DOT agency regulation. No further testing (such as adulteration testing) can be done on the excess urine, and the employee cannot demand that it be turned over to them.